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<110>  ÈÊÑ-×ÅÌ, ÈÍÊ.
 
<120>  ÄÍÊ-ÊÎÄÈÐÎÂÀÍÍÛÅ ÁÈÁËÈÎÒÅÊÈ, ÑÎÄÅÐÆÀÙÈÅ ÑÂßÇÈ ÊÎÄÈÐÓÞÙÈÕ
	ÎËÈÃÎÍÓÊËÅÎÒÈÄÎÂ, ÍÅ ÄÎÑÒÓÏÍÛÅ ÄËß Ñ×ÈÒÛÂÀÍÈß ÏÎËÈÌÅÐÀÇÀÌÈ

<130>  50719-021WO2

<140>  PCT/US2013/050303
<141>  2013-07-12

<150>  61/671,406ô
<151>  2012-07-13

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1
gtgctgc                                                                  7


<210>  2
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2
gcagcaccc                                                                9


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is N-alpha-benzyloxycarbonyl-(Glycine)3-N-succinimidyl ester)

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is OSU (N-succinimidyl ester)

<400>  3

Xaa Gly Gly Gly Gly Gly Gly Xaa 
1               5               


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is azidohomoalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is OSu (N-succinimidyl ester)

<400>  4

Xaa Gly Gly Gly Xaa 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is propargylglycine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Osu (N-succinimidyl ester)

<400>  5

Xaa Gly Gly Gly Xaa 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is an (N-alpha-benzyloxycarbonyl-(Glycine)3-N-succinimidyl 
       ester

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is OSu  (N-succinimidyl ester)

<400>  6

Xaa Gly Gly Gly Xaa 
1               5   


<210>  7
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is CNVK

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  n is CNVK

<400>  7
cgancgtgtc ancg                                                         14


<210>  8
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8
aaaaaagtcg tgacacgtcg gaaaaaaaaa aaacggtgac acggtcgaaa aaa              53


<210>  9
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  n is a 3'-azide (WHS esther)

<400>  9
tgcggtctaa ctgtctaggc acttgttcgt ttgccagtgt gaggaatgaa caggn            55


<210>  10
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-hexynyl

<400>  10
ntaccgaatt gccttcctcg tacagttcta aggcgcttgg acaccacttc aatcgggtgc       60

tatgctt                                                                 67


<210>  11
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  11
tgcggtctaa ctgtcta                                                      17


<210>  12
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12
aagcatagca cccgatt                                                      17


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13
ggcaattcgg tacctgttca ttcc                                              24


<210>  14
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14
ggaatgaaca gg                                                           12


<210>  15
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a 3'-azide (WHS Esther)

<400>  15
tgcggtctaa ctgtctagtt caccttctcc ggaatgaaca ggn                         43


<210>  16
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-Hexynyl

<400>  16
nggaatgaac agggcctgat tgaagcaatc gggtgctatg ctt                         43


<210>  17
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  n is a 3'-azide (WHS Ester)

<400>  17
tgcggtctaa ctgtctaggc acttgttcgt ttgccagtgt gatggacacc acttggaatg       60

aacaggn                                                                 67


<210>  18
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-Hexynyl

<400>  18
nggaatgaac aggggaatga acaggttcct cgtacagttc taaggcgctc aatcgggtgc       60

tatgctt                                                                 67


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19
tgcggtctaa ctgtcta                                                      17


<210>  20
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-Hexynyl

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  n is a 3'-azide (WHS Ester)

<400>  20
nggaatgaac agggtaagct ggagtgaagg cgttatgn                               38


<210>  21
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-dibenzo-cyclooctyne

<400>  21
ngaaggcgtt atgtccgtac tcttgcaatc gggtgctatg ctt                         43


<210>  22
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-dibenzo-cyclooctyne

<400>  22
ngaaggcgtt atgtgatatc cgtggtgtcg tgagttccaa tcgggtgcta tgctt            55


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  23
aaaaaaaaaa gtcgtgac                                                     18


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24
acgtcggtaa aaaaaaaaa                                                    19


<210>  25
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Cyanovinylcarbozole (CNVK)

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is Cyanovinylcarbozole (CNVK)

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  Cyanovinylcarbozole (CNVK)

<400>  25
ncgacgtgtc acgan                                                        15


<210>  26
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5' Psoralen (C2-linked)

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5' Psoralen (C2)

<400>  26
ntagcggatg caaaaaaaaa ggcgagcttg cgtactg                                37


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  27
aaaaaaaaag catccgctct                                                   20


<210>  28
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5' Psoralen (C2-linked)

<400>  28
ngagcggatg caaaaaaaaa ggcgagcttg cgtactg                                37


<210>  29
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  29
tccgctcgac ctgag                                                        15


<210>  30
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  30
aaaaactcag gt                                                           12


<210>  31
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5' biotin

<400>  31
naaaaactca ggt                                                          13


<210>  32
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  32
tccgctcgac ctgag                                                        15


<210>  33
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 56-FAM-Phosphoramidite

<400>  33
nagtacgcaa gctcgc                                                       16


<210>  34
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a monophosphate

<400>  34
ntccgctcga cctgag                                                       16


<210>  35
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  35
tccgctcaac ctgag                                                        15


<210>  36
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36
tccgcttaac ctgag                                                        15


<210>  37
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5-(Carboxy)vinyl-2'-deoxyuridine

<400>  37
ngagcggatg caaaaaaaaa ggcgagcttg cgtactg                                37


<210>  38
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5-(Carboxy)vinyl-2'-deoxyuridine

<400>  38
naagcggatg caaaaaaaaa ggcgagcttg cgtactg                                37


<210>  39
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  n is a 3'-azide (WHS Ester)

<400>  39
tgcggtctaa ctgtctagtt caccttctcc ggaatgaaca ggn                         43


<210>  40
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a 5'-Hexyynyl

<400>  40
ntaccgaatt gccgcctgat tgaagcaatc gggtgctatg ctt                         43


<210>  41
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is a 3'-azide (WHS Ester)

<400>  41
tgcggtctaa ctgtctagtt caccttctcc ggaatgaaca ggn                         43